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Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments
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Biologie
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Journal JoVE Biologie
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

DOI:
10.3791/55034-v

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07:24 min

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December 31, 2016

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, , ,
1Institute of Biochemistry and Molecular Medicine,University of Bern

Chapitres

  • 00:05Titre
  • 00:55Preparation of Xenopus Oocytes
  • 01:45Microinjection of mRNA into Cytoplasm
  • 03:41Strip Follicles
  • 05:15Results: Voltage Clamp Experiment
  • 06:37Conclusion

Summary

Traduction automatique

Traduction automatique

Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.

Tags

Keywords: Xenopus OocytesMicroinjectionFollicular Cell LayersElectrophysiologyIon ChannelsProtein ExpressionGABA A ReceptorMRNACRNAMicroinjection ApparatusPetri DishNylon MeshModified Barth’s Solution

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