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In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
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Journal JoVE Chimie
In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

DOI:
10.3791/55041-v

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14:53 min

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February 03, 2018

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, , , , , , ,
1Centre d’Etudes Nucléaires Bordeaux Gradignan (CENBG),Université de Bordeaux, 2Centre d’Etudes Nucléaires Bordeaux Gradignan (CENBG),CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB),CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB),Université de Bordeaux

Chapitres

  • 00:00Titre
  • 01:38Sample Holder Preparation
  • 03:38Nanoparticles Preparation and Exposure
  • 05:05Paraformaldehyde fixation for Fluorescence Microscopy
  • 05:26Plunge Freezing Fixation and Dehydration
  • 07:13Nuclear Microprobe Analysis
  • 08:58Data Analysis
  • 10:56Representative results
  • 13:12Conclusion

Summary

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We describe a procedure for the detection of chemical elements present in situ in human cells as well as their in vitro quantification. The method is well-suited to any cell type and is particularly useful for quantitative chemical analyses in single cells following in vitro metal oxide nanoparticles exposure.

Tags

NanoparticlesNuclear Microprobe AnalysisSingle Cell QuantificationIn Situ DetectionFluorescence MicroscopyTitanium DioxideCell CulturePEEK Sample HolderPolycarbonate Foil

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