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Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
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Journal JoVE Biologie
Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

DOI:
10.3791/55058-v

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08:17 min

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December 12, 2016

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1Department of Pharmaceutical and Biomedical Sciences,University of Georgia

Chapitres

  • 00:05Titre
  • 00:50Cryopreservation
  • 02:04Collecting Cryosections for RNA Extraction and Histology
  • 04:20RNA Isolation from Pooled Cryosections
  • 06:17Results: RNA Extraction and Histology of Cardiotoxin Injured Muscle
  • 07:40Conclusion

Summary

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Traduction automatique

Consecutive cryo-sections are collected to enable histological applications and enrichment of RNA for gene expression measurements using adjacent regions from a single mouse skeletal muscle. High-quality RNA is obtained from 20 - 30 mg of pooled cryosections and measurements are directly compared across applications.

Tags

Keywords: CryosectioningContiguous RegionsSingle Mouse Skeletal MuscleGene ExpressionHistological AnalysesRegenerative EffectsMuscular InjuryViral Gene DeliveryIntramuscular InjectionHistological DataMolecular DataEmbedding Resin2-MethylbutaneLiquid NitrogenCryostatRNA ExtractionTibialis Anterior

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