Journal
/
/
En mikrofluidsystem med Surface Mønstring for Gransker Kavitasjon Bubble (e)-celle Samhandling og den resulterende bioeffekter på Single-cellenivå
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
DOI:

11:14 min

January 10, 2017

, , , ,

Chapitres

  • 00:05Titre
  • 00:49Gold Dot Patterning
  • 04:19Fabricating the Molecular-assembly Patterning by Lift-off (MAPL)
  • 06:17Chip Assembly and Bubble Investigation
  • 09:11Results: Shear Stress and Membrane Dynamics of Single Cells from Bubble Interactions
  • 10:46Conclusion

Summary

Traduction automatique

A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.

Vidéos Connexes

Read Article