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1Photonics Group, Department of Physics,Imperial College London, 2Institute for Chemical Biology, Department of Chemistry,Imperial College London, 3MRC Clinical Sciences Centre,Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry,Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development,Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology,Imperial College London
Chapitres
- 00:05Titre
- 01:03Open Fluorescence Lifetime Imaging (FLIM)–High Content Analysis (HCA) Plugin Setup
- 01:51Instrument Response Function (IRF) Image and Reference Dye Data Acquisition
- 04:32Multiwell Plate Sample FLIM Data Acquisition
- 06:29Results: Representative FLIM Analyses in Cos and HeLa Cells
- 08:05Conclusion
Vi presentiamo un alto contenuto strumento open source di analisi (HCA) che utilizza l'imaging di fluorescenza vita automatizzato (FLIM) per saggiare le interazioni delle proteine che utilizzano Förster trasferimento di energia di risonanza (FRET) letture basate. L'acquisizione dei dati per questo strumento openFLIM-HCA è controllato da un software scritto in μManager e l'analisi dei dati viene effettuata in FLIMfit.
Tags
Keywords:
Open SourceHigh Content AnalysisAutomatedFluorescence Lifetime Imaging MicroscopyFLIMMulti-well PlateGlobal AnalysisFRETTime-gatedLive Cell ImagingProtein InteractionsCell SignalingMicromanagerOpenFLIM-HCA PluginDelay Box CalibrationInstrument Response FunctionSpinning Disk UnitFast Delay Box
