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Laster et kalsiumfargestoff inn i froskens nerveender gjennom nervestubben: Kalsiumtransientregistrering i Frog Neuromuscular Junction
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

Laster et kalsiumfargestoff inn i froskens nerveender gjennom nervestubben: Kalsiumtransientregistrering i Frog Neuromuscular Junction

DOI:
10.3791/55122-v

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17:05 min

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July 08, 2017

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, , , ,
1Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics,Russian Academy of Sciences, 2Open Laboratory of Neuropharmacology,Kazan Federal University, 3Department of Radiophotonics and Microwave Technologies,A.N. Tupolev Kazan National Research Technical University, 4Department of Medical and Biological Physics,Kazan State Medical University

Chapitres

  • 00:00Titre
  • 00:06Introduction
  • 01:03Preparing of a Dye Loading Solution
  • 01:53Dye Loading Procedure
  • 05:40Praraing the Tissue for Microscopy
  • 08:22Video Capture with Digital CCD Camera
  • 10:06Data Analysis
  • 14:13Résultats
  • 14:56Discussion

Summary

Traduction automatique

Traduction automatique

Her beskriver vi en metode for å legge et kalsiumsensitivt fargestoff gjennom frøens nervestub i nerveenden. Vi presenterer også en protokoll for opptak og analyse av hurtige kalsiumtransienter i perifere nerveender.

Tags

Keywords: Calcium DyeFrog Nerve EndingsNeuromuscular JunctionOptical RecordingCalcium TransientCalcium SignalingHEPESOregon Green BAPTARinger’s SolutionNerve StumpNerve EndingsMuscle Preparation

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