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Дикого типа Блокирование ПЦР в сочетании с прямым секвенированием как высоко чувствительный метод обнаружения низкочастотных соматические мутации
Journal JoVE
Génétique
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Journal JoVE Génétique
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
DOI:

10:41 min

March 29, 2017

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Chapitres

  • 00:05Titre
  • 00:43Wild-Type Blocking PCR: Oligonucleotide Design
  • 03:54Purification of PCR Product by Magnetic Beads
  • 05:53Purification of the Sequencing Products
  • 08:17Identification of MYD88 Mutations in Human Samples
  • 10:03Conclusion

Summary

Traduction automatique

Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

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