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单细胞测序拷贝数改变的检测
Journal JoVE
Génétique
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Journal JoVE Génétique
Detection of Copy Number Alterations Using Single Cell Sequencing
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单细胞测序拷贝数改变的检测

DOI:
10.3791/55143-v

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09:45 min

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February 17, 2017

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, ,
1Koch Institute for Integrative Cancer Research, Department of Biology,Massachusetts Institute of Technology, 2Howard Hughes Medical Institute, 3Division of Health Sciences and Technology,Harvard Medical School, 4The Barbara K. Ostrom (1978) Bioinformatics and Computing Facility in the Swanson Biotechnology Center, Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 5BioMicro Center, Department of Biology,Massachusetts Institute of Technology

Chapitres

  • 00:05Titre
  • 00:48Preparation of Aspirator
  • 04:34Whole Genome Amplification
  • 07:39Results: Detection of Copy Number Alterations Using Single Cell Sequencing
  • 08:48Conclusion

Summary

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单细胞测序是一个日益流行的和可访问在高分辨率解决的基因组变化的工具。我们提供了一个使用单细胞测序，以确定在单个细胞拷贝数变化的协议。

Tags

Single Cell SequencingCopy Number AlterationsGenomic HeterogeneityCytologic ApproachesSensitivitySpecificityMicro-aspiratorSyringe FilterPVC TubingSerological PipetteGlass Capillary TubeWhole Genome AmplificationAdherence CellsTrypsinization10% Bleach70% Ethanol

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