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Opklaringen af ​​funktion en Bakteriel Effector fra et ikke-dyrkbare Plant patogener Ved hjælp af en Gær To hybrid skærm
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Opklaringen af ​​funktion en Bakteriel Effector fra et ikke-dyrkbare Plant patogener Ved hjælp af en Gær To hybrid skærm

DOI:
10.3791/55150-v

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11:30 min

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January 20, 2017

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,
1Department of Molecular Biology – Functional Genomics,Laimburg Research Centre

Chapitres

  • 00:05Titre
  • 00:48Collecting Root and Leaf Samples from Infected Apple Trees
  • 02:22Yeast 2 Hybrid Screen
  • 07:47Analysis of Clones from Selective Plates
  • 08:50Results: Yeast 2 Hybrid to Identify Binding Partners of Bacterial Effector Proteins
  • 09:57Conclusion

Summary

Traduction automatique

Traduction automatique

Bakterielle effektor proteiner er vigtige for at etablere succesfulde infektioner. Denne protokol beskriver den eksperimentelle identifikation af proteinholdige bindingspartnere af en bakteriel effektor-protein i dets naturlige plantevært. Identifikation disse effektor interaktioner via gær to-hybrid-skærme er blevet et vigtigt redskab i optrevling molekylære patogenicitet strategier.

Tags

Bacterial EffectorNon-cultivable Plant PathogenYeast Two-hybrid ScreenCandidatus PhytoplasmaMalus DomesticaApple Proliferation DiseaseInfection ResearchDNA IsolationEffector Gene AmplificationBait VectorSelf-activationEffector ExpressionSD-trp PlateSD-trp MediumNMY51

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