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1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children’s Hospital,University Medical Centre Utrecht, 3Department of Stem Cells and Cancer,Walter and Eliza Hall Institute of Medical Research, 4Hubrecht Institute for Developmental Biology and Stem Cell Research,University Medical Centre Utrecht
Chapitres
- 00:05Titre
- 01:06Colon Organoid Passaging
- 03:56Forskolin-induced Swelling Assay (FIS)
- 05:29Results: Representative FIS Quantification
- 06:14Conclusion
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.
