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여러 형광에 의한 간 염색체 안정 수차의 검출<em> 현장에서</em> 하이브리드 (mFISH) 및 방사선 조사 마우스의 스펙트럼 핵형 분석 (SKY)
Journal JoVE
Génétique
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Journal JoVE Génétique
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
DOI:

10:14 min

January 11, 2017

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Chapitres

  • 00:05Titre
  • 00:53Isolation of Bone Marrow Mononuclear Cells by Density Gradient Centrifugation and Preparation of Metaphase Cell Spreads
  • 03:04Mouse Chromosome Painting by mFISH
  • 06:07Spectral Karyotyping of Mouse Chromosomes
  • 07:45Results: Inter-chromosomal Stable Aberration Analysis by Multiple Fluorescence In Situ Hybridization and Spectral Karyotyping
  • 09:02Conclusion

Summary

Traduction automatique

The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.

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