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Optimierte Analyse von<em> In Vivo</em> und<em> In Vitro</em> Hepatosteatose
Journal JoVE
Médecine
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Journal JoVE Médecine
Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
DOI:

08:58 min

March 11, 2017

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Chapitres

  • 00:05Titre
  • 01:20Tissue Harvesting
  • 01:52Oil Red O Staining and Mounting
  • 04:16Primary Mouse Hepatocyte Model
  • 05:23Purifying Lipids from Liver Tissue
  • 07:18Results: Analysis of Whole Liver Tissue after HFHS Dieting and Analysis of Treated Hepatocyte Cultures
  • 08:26Conclusion

Summary

Traduction automatique

Here, optimized methods to generate in vivo and in vitro models of hepatic steatosis and to analyze the steatotic phenotypes and related physiological parameters are described.

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