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QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
Journal JoVE
Génétique
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Journal JoVE Génétique
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii

DOI:
10.3791/55185-v

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11:37 min

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June 22, 2017

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, ,
1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine,Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine,Louisiana State University

Chapitres

  • 00:05Titre
  • 00:52Assessing SNFr in the Progeny of ME49-FUDRr x VAND-SNFr Cross
  • 02:30CRISPER/Cas9 Editing: Inducing Mutations
  • 06:20CRISPER/Cas9 Editing: Examining Mutations
  • 08:33Results: QTL Mapping and CRISPER/Cas9 Editing Identified SNR1 as the Sinefungin Resistance Gene
  • 10:29Conclusion

Summary

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Traduction automatique

Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.

Tags

Keywords: QTL MappingCRISPR/Cas9Drug ResistanceToxoplasma GondiiSinefunginWhole Genome SequencingSNP AnalysisGenetic ManipulationParasite Growth AssayQuantitative Trait LocusGenetic BasisPathogenesis

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