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Метод тампон Отбор проб для обнаружения человека норовирус на поверхностях
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
DOI:

10:03 min

February 06, 2017

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Chapitres

  • 00:05Titre
  • 01:04Swab Sampling, Virus Concentration, Viral RNA Extraction, and Purification
  • 02:36Midi Column Viral Nucleic Acid Extraction
  • 03:40Viral Nucleic Acid Concentration
  • 05:16Multiplex RT-qPCR Detection
  • 07:09Quantification of Norovirus in Swab Samples and Hemi-nested Conventional PCR Amplification
  • 08:11Results: Macrofoam-based Swab Sampling Method Effectively Detects Norovirus Outbreaks
  • 09:24Conclusion

Summary

Traduction automatique

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

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