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Bağışıklık ilaçlarının tanımlanmasında T lenfositlerinde mitojen kaynaklı blastojenez hızlı bir şekilde belirlenmesini
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Chapitres
- 00:05Titre
- 00:57Raw Splenocyte Preparation
- 02:36T Lymphocyte Purification, Activation, and Experimental Drug Exposure
- 04:41Automated Cell Counter Data Collection
- 05:40Results: Representative Effects of T Cell Stimuli and Immunosuppressants on Cell Diameter and Proliferation
- 07:11Conclusion
T-lymphocyte mitogenesis is accompanied by blastogenic transformation, whereupon the cell volume enlarges before cell division. Here, we describe a method to quantify blastogenesis in T lymphocytes using an automated cell counter with the capability of measuring cell diameters.
