Journal
/
/
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
DOI:

10:15 min

March 22, 2017

, , ,

Chapitres

  • 00:05Titre
  • 01:213T3 Feeder Cell Culture and Irradiation
  • 02:31Pediatric Esophageal Biospy Acquisition and Processing
  • 05:00Cell Culture Expansion, Freezing, and Storage
  • 07:17Results: Representative Human Esophageal Cell Characterization
  • 08:59Conclusion

Summary

Traduction automatique

Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.

Vidéos Connexes

Read Article