Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:46Generation and Purification of PCR Products Corresponding to Two Mutated and Partially Overlapping Histone Gene Fragments
- 02:06Fusion PCR of the Two Partially Overlapping Fragments to Generate Full Size PCR Products for Integration
- 03:20Co-transformation, Screen for 5-FOA-resistant Transformants, Purification of 5-FOA-resistant Colonies, and Loss of Backbone Plasmid
- 05:11Results: In Situ Mutagenesis of HHT2 in Yeast
- 07:09Conclusion
