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A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines

A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines

DOI:
10.3791/55271-v

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07:59 min

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March 04, 2017

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, , , , ,
1Department of Radiology,Memorial Sloan Kettering Cancer Center, 2Translational and Molecular Imaging Institute,Icahn School of Medicine at Mount Sinai

Chapitres

  • 00:05Titre
  • 00:56Synthesis of Dual-labeled Liposomal Nanoparticles
  • 03:22In Vivo PET-CT Imaging and Ex Vivo Biodistribution Measurements
  • 06:19Results: Biodistribution of Fluorescent- and Radiolabeled Nanoparticles in Tumor-bearing Mice
  • 07:14Conclusion

Summary

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The poor understanding of the in vivo performance of nanomedicines stymies their clinical translation. Procedures to evaluate the in vivo behavior of cancer nanomedicines at systemic, tissue, single-cell, and subcellular levels in tumor-bearing immunocompetent mice are described here. This approach may help researchers to identify promising cancer nanomedicines for clinical translation.

Tags

Keywords: Cancer NanomedicinesIn Vivo PerformanceTumor-bearing MiceDual-labeling ApproachLiposomesLipophilic Cationic Fluorescent DyePhosphate-buffered SalineDynamic Light ScatteringRadiolabelingZirconium-89 OxalateSize-exclusion HPLCRadiochemical Purity

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