Journal
/
Recherche en cancérologie
/
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation
Journal JoVE
Recherche en cancérologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Recherche en cancérologie
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation

A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation

DOI:
10.3791/55279-v

•

09:23 min

•

February 13, 2017

•

, ,
1Department of Oncology,Lombardi Comprehensive Cancer Center, 2Department of Oncology,Georgetown University Medical Center

Chapitres

  • 00:05Titre
  • 00:52Establishment of the 3D Cell Culture Insert System
  • 01:28Processing the Filters for Pellet Banking
  • 03:17Processing the Filters for RNA or DNA Isolation
  • 04:20Processing the Filters for Protein Isolation
  • 05:42Processing for H&E and Immunostaining
  • 07:42Results: Representative Bright Field and Immunofluorescence Images of the Cells Sectioned on the Filter
  • 08:22Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.

Tags

Keywords: 3D CulturePrimary Prostate CellsProstate Cancer ResearchIn Vitro ModelFilter InsertCell DifferentiationRNADNAProteinGelatin CoatingTrypsin EDTAConditioned MediaCentrifugation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/55279?article_type=v

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)
check_url/fr/55279?article_type=v

Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
check_url/fr/55279?article_type=v

Therapy Testing in a Spheroid-based 3D Cell Culture Model for Head and Neck Squamous Cell Carcinoma
check_url/fr/55279?article_type=v

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
check_url/fr/55279?article_type=v

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
check_url/fr/55279?article_type=v

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
check_url/fr/55279?article_type=v

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
check_url/fr/55279?article_type=v

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures
check_url/fr/55279?article_type=v

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
check_url/fr/55279?article_type=v

Differentiation of Mouse Breast Epithelial HC11 and EpH4 Cells

Read Article