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Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
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Journal JoVE Biologie
Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

DOI:
10.3791/55324-v

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07:40 min

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February 05, 2018

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,
1Environmental and Conservation Sciences Program,North Dakota State University, 2Department of Biological Sciences,North Dakota State University

Chapitres

  • 00:04Titre
  • 00:41Semen Collection from a Passerine Bird Using Cloacal Massage
  • 03:34Extraction of DNA from Bird Semen
  • 06:32Results: Success by Bird Species and DNA Electrophoresis
  • 07:08Conclusion

Summary

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Traduction automatique

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

Tags

Avian Semen CollectionCloacal MassageDNA IsolationSpermBehavioral EcologyEvolutionary EcologySperm CompetitionEpigenetic InheritanceField CollectionCaptive BirdsBreeding SeasonCloaca EversionSemen ExtractionMicrohematocrit TubeSperm VisualizationSample Storage

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