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Chapitres
- 00:05Titre
- 00:49Collection and Preparation of the Colon
- 02:30Colon Organ Culture
- 04:43E. coli Killing Assay
- 06:26Effect of Extrinsic and Intrinsic Factors on Colonic Antimicrobial Host Defense Responses
- 08:51Results: Intestinal Antimicrobial Immune Responses in Ex Vivo Cultured Colon
- 10:11Conclusion
The ex vivo organ culture allows investigation of biological processes in the context of the intact tissue architecture. Here, we introduce a method of ex vivo culture of the mouse colon, which can be used to study innate immunity and antimicrobial host defense in the intestine.
Tags
Ex VivoColon Organ CultureAntimicrobial Host DefensePathogen Associated Molecular PatternsPattern Recognition ReceptorsInnate Immune PathwaysAntimicrobial PeptidesIntestinal ArchitectureEuthanasiaPeritoneumCecumRectumPBSDMEM F12FBSPenicillin StreptomycinGentamycinCell Strainer12-well Cell Culture Plate
