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Organotypic hippocampus skive kulturer som en modell å studere Neuroprotection og Invasiveness av kreftceller
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Neurosciences
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Journal JoVE Neurosciences
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Organotypic hippocampus skive kulturer som en modell å studere Neuroprotection og Invasiveness av kreftceller

DOI:
10.3791/55359-v

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07:48 min

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August 27, 2017

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, , ,
1Department of Anatomy and Cell Biology,Martin Luther University Halle-Wittenberg, 2Department of Anatomy,University of Otago

Chapitres

  • 00:00Titre
  • 00:08Agar Preparation
  • 00:54Brain Fixation
  • 02:29Slicing Procedure
  • 03:26Hippocampus Removal
  • 04:05Culturing Method
  • 05:18Representative Results
  • 07:19Conclusion

Summary

Traduction automatique

Traduction automatique

Organotypic hippocampus skive kulturer (OHSC) representerer en i vitro modell som simulerer i vivo situasjonen veldig godt. Her beskriver vi en vibratome-basert forbedret kutting protokoll for å få høy kvalitet skiver for bruk i vurderingen neuroprotective potensialet i romanen stoffer eller biologisk atferd av kreftceller.

Tags

Keywords: Organotypic Hippocampal Slice CulturesNeuroprotectionTumor Cell InvasivenessVibratomeAgar BlocksPreparation MediumCytoarchitectureCell Culture InsertCulture MediumInflammatory ReactionsNeuroprotection Studies

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