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Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation
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Journal JoVE Biologie du développement
Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

DOI:
10.3791/55373-v

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10:11 min

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May 06, 2017

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1Toronto General Research Institute,University Health Network, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto, 3Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, 4Laboratory of Medicine and Pathobiology,University of Toronto

Chapitres

  • 00:05Titre
  • 01:01Fabrication of Biowire Templates
  • 02:22Fabricating the Electrical Simulation Chambers
  • 03:55Gel Preparation and Seeding of Cardiomyocytes into the Template
  • 05:41Culturing Cardiomyocytes in the Template with Electrical Stimulation (Biowire)
  • 07:22Results: Biowire Cardiomyocyte Physiology
  • 09:16Conclusion

Summary

Traduction automatique

Traduction automatique

The cardiac biowire platform is an in vitro method used to mature human embryonic and induced pluripotent stem cell-derived cardiomyocytes (hPSC-CM) by combining three-dimensional cell cultivation with electrical stimulation. This manuscript presents the detailed setup of the cardiac biowire platform.

Tags

Keywords: Stem CellCardiomyocyteMaturationElectrical StimulationBiowireDrug ScreeningPDMSSuturePolycarbonateCarbon RodPlatinum Wire

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