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Forberedelse af meiotiske kromosom spreder sig fra musen Spermatocytes
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Journal JoVE Biologie
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

Forberedelse af meiotiske kromosom spreder sig fra musen Spermatocytes

DOI:
10.3791/55378-v

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06:38 min

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November 22, 2017

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, , , ,
1Department of Biochemistry & Molecular Biology,Drexel University College of Medicine, 2Department of Obstetrics & Gynecology,Drexel University College of Medicine

Chapitres

  • 00:05Titre
  • 00:52Spermatocyte Nuclei Spreading
  • 04:44Results: Representative Wild-type, Juvenile Mouse, Surface-Spread Nuclei
  • 05:32Conclusion

Summary

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Traduction automatique

Meiose er den udviklingsmæssige proces hvorved mælke dannes ved en enkelt runde af DNA-replikation og to efterfølgende runder af kromosom adskillelse. Pattedyr meiose kan undersøges ved at benytte en teknik til at forberede meiotiske kromosom spreads. Her viser vi en metode til at forberede overflade-spredning kerner fra mus spermatocytes.

Tags

Meiotic Chromosome SpreadsMouse SpermatocytesReproductive BiologyMeiotic RecombinationChromosome SegregationSeminiferous TubulesHypotonic Extraction BufferFixative SolutionTeflon Printed Slides

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