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Immunology and Infection
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JoVE Journal Immunology and Infection
Strømlinet enkelt celle TCR Isolation og Generation af Retroviral vektorer for In Vitro og In Vivo udtryk for menneskelige TCRs
 
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Strømlinet enkelt celle TCR Isolation og Generation af Retroviral vektorer for In Vitro og In Vivo udtryk for menneskelige TCRs

Article DOI: 10.3791/55379-v • 11:21 min • September 10th, 2017
September 10th, 2017 •
1, 2, 1, 1, 3, 2, 4, 1, 1
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital

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De nuværende protokol kombinerer enkelt celle parret menneskelige TCR alfa og beta kæde sekventering med strømlinet generation af retroviral vektorer kompatibel med in vitro- og i vivo TCR udtryk.

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Immunologi sag 127 T celle T celle receptoren TCR PCR sekventering retroviral enkelt celle menneskelige in vivo, in vitro- TCR udtryk
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