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Immunology and Infection
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JoVE Journal Immunology and Infection
Aerodinâmico única célula TCR isolamento e geração de vetores retrovirais para In Vitro e In Vivo de expressão de TCRs humanas
 
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Aerodinâmico única célula TCR isolamento e geração de vetores retrovirais para In Vitro e In Vivo de expressão de TCRs humanas

Article DOI: 10.3791/55379-v • 11:21 min • September 10th, 2017
September 10th, 2017 •
1, 2, 1, 1, 3, 2, 4, 1, 1
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital

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O atual combina de protocolo único celular emparelhado humana TCR alfa e beta cadeia sequenciamento com simplificada geração de vetores retrovirais compatíveis com expressão de TCR in vitro e in vivo .

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Imunologia edição 127 células T T cell receptor TCR PCR sequenciamento célula retroviral única humana vivo em, em vitro expressão de TCR
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