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Physiological Preparation of Hair Cells from the Sacculus of the American Bullfrog (Rana catesbeiana)
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Chapitres
- 00:05Titre
- 00:58Extraction of Inner Ear Organs
- 03:52Isolation of Sacculus
- 06:03One-chamber Preparation: Digestion and Mounting
- 06:51Two-chamber Preparation: Digestion and Mounting
- 10:08Results: Displacement-response and Hair Bundle Oscillation
- 11:18Conclusion
The American bullfrog's (Rana catesbeiana) sacculus permits direct examination of hair-cell physiology. Here the dissection and preparation of the bullfrog's sacculus for biophysical studies is described. We show representative experiments from these hair cells, including the calculation of a bundle's force-displacement relation and measurement of its unforced motion.
