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Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure
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Médecine
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Journal JoVE Médecine
Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

Absolute Quantification of Aβ1-42 in CSF Using a Mass Spectrometric Reference Measurement Procedure

DOI:
10.3791/55386-v

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08:40 min

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March 21, 2017

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, , ,
1Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, 2Clinical Neurochemistry Laboratory,Sahlgrenska University Hospital, 3UCL Institute of Neurology, Queen Square

Chapitres

  • 00:05Titre
  • 01:13Preparation of the Calibrators and the Internal Standard
  • 03:00Preparation of the Response Factor Sample
  • 04:01Sample Preparation
  • 05:06Solid Phase Extraction
  • 06:32Results: Quantification of Aβ1-42 in CSF Using Solid Phase Extraction and LC-MS/MS
  • 07:25Conclusion

Summary

Traduction automatique

Traduction automatique

A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is described.

Tags

Absolute QuantificationA-beta 1-42CSFMass Spectrometric Reference Measurement ProcedureAlzheimer’s DiseaseBiomarkerAmyloid PathologyTransgenic MiceNon-human PrimatesHydrophobicCalibrator SolutionsInternal StandardResponse Factor Sample

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