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Analyse av lymfocytt-ekstravasasjon ved hjelp av en<em> In Vitro</em> Modell av det menneskelige blod-hjernebarrieren
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Immunologie et infection
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Journal JoVE Immunologie et infection
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier

Analyse av lymfocytt-ekstravasasjon ved hjelp av en<em> In Vitro</em> Modell av det menneskelige blod-hjernebarrieren

DOI:
10.3791/55390-v

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09:00 min

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April 05, 2017

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, , , , ,
1Department of Neurology with Institute of Translational Neurology,University Hospital Münster

Chapitres

  • 00:05Titre
  • 00:55Human Brain Microvascular Endothelial Cell (HBMEC) Culture
  • 02:26Transmigration Assay
  • 05:47Flow Cytometry Data Analysis
  • 07:01Results: Differential NK Cell Subset Migration across Inflamed and Control HBMEC Monolayers
  • 08:07Conclusion

Summary

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Traduction automatique

Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.

Tags

Lymphocyte ExtravasationIn Vitro ModelBlood-brain BarrierInflammatory DiseasesCentral Nervous SystemTransmigration AssayEvans Blue PermeationHBMECFibronectinAccutaseCell Culture Inserts

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