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Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells
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Biologie
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Journal JoVE Biologie
Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

Using Microfluidic Devices to Measure Lifespan and Cellular Phenotypes in Single Budding Yeast Cells

DOI:
10.3791/55412-v

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09:18 min

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March 30, 2017

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, , , ,
1The State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, School of Physics,Peking University, 2Department of Biochemistry and Biophysics,University of California, San Francisco, 3Peking-Tsinghua Center for Life Sciences at Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies,Peking University

Chapitres

  • 00:05Titre
  • 01:22Microfluidic Device Molding
  • 03:04Microfluidic Device Preparation and Assembly
  • 04:56Microfluidic Tube Preparation
  • 06:12Loading of a Yeast Sample
  • 07:28Results: Fresh Mother Cells Live Longer than Cells with Unknown History
  • 08:27Conclusion

Summary

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Traduction automatique

This article presents a protocol optimized for the production of microfluidic chips and the setup of microfluidic experiments to measure the lifespan and cellular phenotypes of single yeast cells.

Tags

Keywords: Microfluidic DevicesLifespanCellular PhenotypesSingle Budding Yeast CellsYeast AgingCell TrackingMolecular MarkersSecondary ResponsesCell LineagePDMSSilicon Wafer MoldDevice FabricationCell LoadingMicroscope Setup

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