Journal
/
/
Live-Imaging af antifungal aktivitet af humane primære neutrofiler og monocytter i Reaktion på<em> A. fumigatus</em
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
DOI:

12:29 min

April 19, 2017

, , , , , ,

Chapitres

  • 00:05Titre
  • 00:54Aspergillus fumigatus Culture and Labeling
  • 04:28Isolation of Human Monocytes
  • 07:30Isolation of Human Neutrophils
  • 08:30Live Cell Video Microscopy
  • 09:31Results: In Vitro Evaluation of Primary Immune Response to Aspergillus Conidia
  • 11:11Conclusion

Summary

Traduction automatique

Her beskriver vi en protokol til at vurdere antifungal aktivitet af primære humane immunceller i realtid under anvendelse af fluorescerende Aspergillus reporter konidier sammenholdt med live-cell video mikroskopi og flowcytometri. Genererede data give indsigt i værtens celle- Aspergillus interaktioner såsom fungicid aktivitet, fagocytose, cellevandring og hæmning af svampevækst.

Vidéos Connexes

Read Article