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Reinigung von High Molecular Weight genomischer DNA aus Echten Mehltaus für Long-Read-Sequenzierung
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Génétique
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Journal JoVE Génétique
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
DOI:

06:56 min

March 31, 2017

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Chapitres

  • 00:05Titre
  • 00:32Genomic DNA Purification
  • 01:22DNA Extraction I and Precipitation I
  • 03:30DNA Extraction II and Precipitation II
  • 05:44Results: Gels Distinguish Acceptable, Marginal and Failed Genomic DNA Samples
  • 06:33Conclusion

Summary

Traduction automatique

Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.

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