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遗传编码的荧光一氧化氮的应用(NO•)探针,所述geNOps,用于在单细胞NO•信号的实时影像
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
DOI:

08:32 min

March 16, 2017

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Chapitres

  • 00:05Titre
  • 01:07Cell Preparation
  • 02:33Live Cell Imaging of NO• and Ca2+ Signals in Individual Cells
  • 06:28Results: Simultaneous Multichannel Imaging of NO• and Ca2+ Signals in Single EA.hy926 Cells
  • 07:50Conclusion

Summary

Traduction automatique

This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.

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