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유전자 인코딩 된 형광 산화 질소의 응용 프로그램 (• NO) 단일 세포에서 NO • 신호의 실시간 이미징을위한 프로브의 geNOps,
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
DOI:

08:32 min

March 16, 2017

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Chapitres

  • 00:05Titre
  • 01:07Cell Preparation
  • 02:33Live Cell Imaging of NO• and Ca2+ Signals in Individual Cells
  • 06:28Results: Simultaneous Multichannel Imaging of NO• and Ca2+ Signals in Single EA.hy926 Cells
  • 07:50Conclusion

Summary

Traduction automatique

This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.

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