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Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
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Biochimie
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Journal JoVE Biochimie
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

DOI:
10.3791/55502-v

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10:44 min

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March 28, 2017

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1Research and Development,British American Tobacco

Chapitres

  • 00:05Titre
  • 00:48Cell Culture
  • 02:49CYP Activity Quantification in Cell Cultures: Metabolic Probes, Inducers, and Inhibitors
  • 04:30CYP Activity Quantification in Cell Cultures: Medium Treatment
  • 05:40CYP Activity Quantification in Cell Cultures: Mass Spectrometry (MS)-based Quantification
  • 06:49CYP Activity Quantification in Cell Cultures: Probes Using Luminescence-based Methods
  • 08:10Results: Quantification of Metabolites Produced by tha Activity of CYP Enzymes Following Cell Incubation with CYP Inducers, Substrates, and Inhibitors
  • 09:35Conclusion

Summary

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Traduction automatique

Metabolic competency of in vitro systems is a key requirement for the biotransformation and disposition of drugs and toxicants. In this protocol, we describe the application of reference metabolic probes to assess phase I metabolism in cell cultures.

Tags

Keywords: Mass SpectrometryLuminogenicPhase I MetabolismIn Vitro Cell CulturesCytochrome P450CYP EnzymesProbe SubstratesInhibitorsHepatic Cell LineWilliams’ E MediumAir-liquid InterfaceTransepithelial ResistanceCilia Beat Frequency

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