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En allsidig Måte mønster Proteiner og celler
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Journal JoVE Bio-ingénierie
A Versatile Method of Patterning Proteins and Cells

En allsidig Måte mønster Proteiner og celler

DOI:
10.3791/55513-v

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09:57 min

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February 26, 2017

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, , , , ,
1Department of Biomedical Engineering,Rutgers University, 2Department of Cell Biology and Neuroscience,Rutgers University, 3Department of Biomedical Engineering,New Jersey Institute of Technology, 4Department of Pharmacology, Physiology, and Neuroscience,Rutgers New Jersey Medical School

Chapitres

  • 00:05Titre
  • 01:00Fabrication of Master Molds for Soft Lithography Using Adhesive Tape
  • 02:32Soft Lithography Fabrication of PDMS Devices
  • 04:02Substrate Patterning
  • 05:22Indirect Patterning of Cells
  • 06:29Direct Patterning of Cells
  • 08:05Results: Direct and Indirect Patterning of Cells
  • 08:56Conclusion

Summary

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Traduction automatique

This report describes a simple, easy to perform technique, using low pressure vacuum, to fill microfluidic channels with cells and substrates for biological research.

Tags

Keywords: PatterningProteinsCellsMicrochannelsPDMSAdhesive TapeGlass SlideCAD DrawingIsopropanolAir GunOvenRubber RollerElastomerCuring AgentVacuum ChamberScalpelTweezersBiopsy PunchCell-to-cell InteractionsCell-to-protein Interactions

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