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Un metodo delle celle di aggregazione efficiente e flessibile per il 3D Spheroid Produzione
Journal JoVE
Biologie
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Journal JoVE Biologie
An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production
DOI:

07:46 min

March 27, 2017

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Chapitres

  • 00:05Titre
  • 00:40Spheroid Production by Cell Aggregation
  • 03:44Example Application: Embedding Spheroids in Collagen
  • 05:30Results: Representative Spheroid Formation
  • 06:54Conclusion

Summary

Traduction automatique

Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.

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