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Immunologie et infection
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Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

DOI:
10.3791/55570-v

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09:31 min

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April 15, 2017

•

,
1WHO Collaborating Centre for Reference and Research on Influenza,Peter Doherty Institute for Infection and Immunity

Chapitres

  • 00:05Titre
  • 01:00Determining the Neuraminidase Activity of Viruses
  • 02:29Assessing Virus Seceptibility to NA Inhibitors Using the NA Inhibition Assay
  • 05:18Calculation of IC50 Values
  • 07:01Results: Susceptibility of Influenza Viruses to the Neuraminidase Inhibitor Class of Antivirals
  • 08:55Conclusion

Summary

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We describe the use of a phenotypic fluorescence-based neuraminidase inhibition assay to assess the susceptibility of influenza A and B viruses to the neuraminidase inhibitor class of antivirals.

Tags

Fluorescence-based AssayNeuraminidase InhibitionInfluenza Virus SusceptibilityNeuraminidase Inhibitor AntiviralsMUNANAVirus TitrationNA Activity CurvesZanamivirOseltamivir CarboxylatePeramivirLaninamivir

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