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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

DOI:
10.3791/55605-v

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12:00 min

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July 05, 2017

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, , , , ,
1Department of Dietetics and Nutrition,University of Arkansas for Medical Sciences, 2Department of Nutrition, Food and Exercise Sciences,Florida State University, 3Center for Advancing Exercise and Nutrition Research on Aging (CAENRA),Florida State University

Chapitres

  • 00:05Titre
  • 01:08Preparation of Crude Blackberry Extracts
  • 03:31Preparation of Polyphenol Extracts from Crude Berry Extracts
  • 05:13Treatment of Vascular Smooth Muscle Cells with Berry Extracts-purified Polyphenols
  • 09:08Results: Polyphenol Composition and Phosphorylation Reduction by Crude and Purified Berry Extracts
  • 11:05Conclusion

Summary

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This work details a step-by-step method to prepare polyphenol-rich extracts from freeze-dried berry powder. In addition, it provides a thorough description of how to use these polyphenol-rich extracts in cell culture in the presence of the peptide hormone angiotensin II (Ang II) using Vascular Smooth Muscle Cells (VSMCs).

Tags

Polyphenol ExtractionFreeze-dried Berry PowderUltrasonic-assisted ExtractionEthanol-based ExtractionChloroform PurificationEthyl Acetate FractionationVascular Smooth Muscle CellsIn Vitro Treatment

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