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High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
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Biologie
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Journal JoVE Biologie
High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

DOI:
10.3791/55610-v

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10:23 min

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April 20, 2017

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, ,
1Department of Cell and Developmental Biology,University of Colorado School of Medicine

Chapitres

  • 00:05Titre
  • 00:38Growing Liquid Yeast Culture
  • 01:26Preparing Flow Chamber Slides
  • 03:36Loading Yeast Cells into Prepared Flow Chamber Slides
  • 05:03Image Acquisition
  • 06:30Image Analysis
  • 08:15Results: Astral Microtubule Dynamics in Altered in tub2-430Δ Mutant Cells
  • 09:32Conclusion

Summary

Traduction automatique

Traduction automatique

Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.

Tags

Keywords: Microtubule DynamicsYeast CellsGFP-labeled TubulinFlow Chamber SlidesParaffin FilmTime-lapse ImagingMicroscopyCell BiologyCytoskeleton

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