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腹腔镜技术在猕猴肝和肠系膜淋巴结的串行采集

Journal JoVE
Médecine

This content is Free Access.

Journal JoVE Médecine

Laparoscopic Technique for Serial Collection of Liver and Mesenteric Lymph Nodes in Macaques

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腹腔镜技术在猕猴肝和肠系膜淋巴结的串行采集

DOI:

10.3791/55617-v

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11:06 min

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May 02, 2017

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Alexander S. Zevin, Cassie Moats, Drew May, Solomon Wangari, Charlene Miller, Joel Ahrens, Naoto Iwayama, Megan Brown, Debbie Bratt, Nichole R. Klatt, Jeremy Smedley

¹Department of Pharmaceutics,Washington National Primate Research Center, University of Washington, ²Division of Primate Resources,Washington National Primate Research Center, University of Washington

Chapitres

00:05Titre
01:12Pre-surgical Preparation
01:49Laparoscopic Entry and Cannula Placement
04:17Mesenteric Lymph Node Biopsy
06:38Liver Biopsy
07:53Results: Identification of Leukocyte and Lymphocyte Populations in Mesenteric Lymph Node and Liver Biopsies
09:24Conclusion

Summary

Traduction automatique

在这里，我们描述了用于在猕猴肝脏和肠系膜淋巴结（MLN）的连续取样，可以增加采样频率，并与执行剖腹时减少了手术并发症的潜力微创腹腔镜技术。

Tags

Keywords: Laparoscopic Technique Serial Collection Liver Mesenteric Lymph Nodes Macaques Minimally Invasive GI Tract Microbial Translocation HIV Biology Immunological Consequences Pathology Multiple Time Points Animal Welfare Sterile Procedure Scalpel Incision Varus Needle Pneumoperitoneum CO2 Insufflator

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