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Biologie du développement
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Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
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Biologie du développement
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Journal JoVE Biologie du développement
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

DOI:
10.3791/55630-v

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10:45 min

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May 25, 2017

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1Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center, 2Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center

Chapitres

  • 00:05Titre
  • 00:50Acute Ethanol Treatment in Zebrafish Larvae
  • 01:57Preparation of Tissue Cassettes and Processing
  • 04:02Tissue Sectioning
  • 05:49Hematoxylin and Eosin (H&E) Staining of Paraffin-embedded Sections
  • 08:44Results: Comparisons of H&E Staining in the Livers of Zebrafish Larvae
  • 10:14Conclusion

Summary

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This protocol describes histological analyses of the livers from zebrafish larvae that have been treated with 2% ethanol for 24 h. Such acute ethanol treatment results in hepatic steatosis and swelling of the hepatic vasculature.

Tags

ZebrafishHistologyAcute Alcoholic Liver InjuryPatho-histologyEthanolAlcohol Liver Disease2% EthanolDietrich’s FixativePBS3% AgaroseSagittal Sections

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