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Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
DOI:

09:57 min

May 10, 2017

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Chapitres

  • 00:05Titre
  • 01:41Cell Preparation
  • 02:51Sample Preparation and Staining
  • 05:44Flow Cytometry Analysis
  • 07:24Results: Identification and Characterization of Side Population Cells
  • 08:20Conclusion

Summary

Traduction automatique

Methods allowing the characterization and isolation of stem cell populations from biological samples are critical for the advance of stem cell-targeted treatments in cancer and beyond. Here, we provide a detailed protocol for cancer stem cell isolation using the dye-triggered side population phenotype.

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