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Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells
Journal JoVE
Biologie
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Journal JoVE Biologie
Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

Mixed Primary Cultures of Murine Small Intestine Intended for the Study of Gut Hormone Secretion and Live Cell Imaging of Enteroendocrine Cells

DOI:
10.3791/55687-v

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09:16 min

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April 20, 2017

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, , , ,
1Metabolic Research Laboratories and MRC Metabolic Diseases Unit,Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge

Chapitres

  • 00:05Titre
  • 00:54Tissue Preparation
  • 03:15Removal of Cell Debris and Single Cells
  • 04:44Collection of Crypt Fragments
  • 06:55Intestinal Culture Plating
  • 07:38Results: Secretion and Imaging Using Primary Small Intestinal Cultures
  • 08:36Conclusion

Summary

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This protocol describes the isolation and culture of mixed murine small intestinal cells. These primary intestinal cultures enable the investigation of signaling pathways underlying gut peptide secretion using a number of techniques.

Tags

Keywords: Enteroendocrine CellsGut Hormone SecretionLive Cell ImagingMixed Primary CulturesMurine Small IntestineIntestinal EpitheliumIntestinal ContentsMuscle LayerEpitheliumChymeMucus

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