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Ex Vivo Avbildning av bosatta CD8 T-lymfocyter i Human Lung tumör skivor med konfokalmikroskopi
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy
DOI:

08:28 min

December 27, 2017

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Chapitres

  • 00:05Titre
  • 01:01Agarose Embedding and Vibratome Slicing of Human Lung Tumors
  • 03:51Immunostaining of Resident CD8 T Cells and Tumor Cells
  • 05:07Imaging and Analyzing Resident CD8 T Cell Migration Within Human Lung Tumor Slices
  • 07:03Results: Distribution and Motility Patterns of Resident CD8 T Cells in Human Lung Tumor Tissues
  • 07:53Conclusion

Summary

Traduction automatique

Det här protokollet beskriver en metod för att bilden bosatt tumör-infiltrera CD8 T-celler märkta med fluorescently kopplat antikroppar inom human lung tumör skivor. Denna teknik tillåter realtid analyser av CD8 T cellmigration med konfokalmikroskopi.

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