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Isolering af Side Population i Myc-induceret T-celle akut lymfoblastisk leukæmi i zebrafisk
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of the Side Population in Myc-induced T-cell Acute Lymphoblastic Leukemia in Zebrafish

Isolering af Side Population i Myc-induceret T-celle akut lymfoblastisk leukæmi i zebrafisk

DOI:
10.3791/55711-v

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09:06 min

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May 04, 2017

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, , ,
1Department of Pediatrics, Section of Hematology-Oncology,The University of Chicago, 2Department of Biology and Biotechnology,Worcester Polytechnic Institute

Chapters

  • 00:05Title
  • 01:07Isolation of Fluorescently Labeled T-ALL Cells from Zebrafish
  • 04:01Identification of Side Population Cells in Zebrafish T-ALL
  • 06:53Results: Side Population Identification in T-ALL Cells from Zebrafish
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en teknik til isolering af sidebefolkningscellerne fra en zebrafiskmodel af myc-induceret T-celle akut lymfoblastisk leukæmi (T-ALL). Denne sidebestemmelsesanalyse er meget følsom og er beskrevet for zebrafisk T-ALL, men den kan være anvendelig for andre maligne og ikke-maligne zebrafiskcelletyper.

Tags

Side PopulationT-cell Acute Lymphoblastic LeukemiaZebrafishMyc-induced CancerCancer Stem CellsCell IsolationHeparinEuthanasiaFBS/PBSCell Collection

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