Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:55Mouse Heart Isolation and Ventricle Embedding
- 02:30Slicing the Ventricle Tissue
- 03:47Sharp Electrode Setup Preparation and Action Potential Recordings
- 05:51Results: Electrical Integration of Transplanted Induced Pluripotent Stem Cell iPSCM
- 06:45Conclusion
Here, we describe the preparation of viable ventricular slices from adult mice and their use for sharp electrode action potential recordings. These multicellular preparations provide a preserved in vivo like tissue structure, which makes them a valuable model for electrophysiological and pharmacological studies in vitro.
