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The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy

The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy

DOI:
10.3791/55737-v

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07:55 min

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July 09, 2017

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, ,
1Research Laboratory for Stereology and Neuroscience,Bispebjerg-Frederiksberg Hospital, 2Institute of Clinical Medicine, Department of Health and Medical Sciences,University of Copenhagen

Chapitres

  • 00:05Titre
  • 00:52Brain Tissue Processing
  • 02:11Immunostaining
  • 04:49Estimation of Total Number of BrdU-labeled Neurons Using the Optical Fractionator
  • 06:46Results: Total Number of BrdU-positive Neurons in the GCL-SGZ of the Rat Hippocampus
  • 07:32Conclusion

Summary

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Traduction automatique

Here, we present a stereological method, the optical fractionator, used to quantify the formation of new neurons, and their survival, in the rat hippocampus following electroconvulsive stimulation. When correctly implemented, the sensitivity and efficiency of stereological methods ensures accurate estimates with a fixed and predetermined precision.

Tags

Keywords: Optical FractionatorStereological StudyCell Number EstimationRat ModelElectroconvulsive TherapyQuantitative AnalysisBrain AreaUnbiased SamplingFixed PrecisionBrain HemispheresHippocampusImmunostainingCryostat SectioningCryoprotectant

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