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DiI Perfusion som en metode til vaskulær visualisering i<em> Ambystoma mexicanum</em
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Journal JoVE Biologie
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum

DiI Perfusion som en metode til vaskulær visualisering i<em> Ambystoma mexicanum</em

DOI:
10.3791/55740-v

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06:05 min

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June 16, 2017

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, , , ,
1Department of Orthopedic Surgery,Harvard Medical School and Brigham and Women’s Hospital, 2Division of Graduate Medical Sciences,Boston University

Chapitres

  • 00:05Titre
  • 00:50Axolotl Chest Opening
  • 02:04Axolotl Perfusion and Vasculature Visualization
  • 04:34Results: Representative Fully Perfused Axolotl Imaging
  • 04:59Conclusion

Summary

Traduction automatique

Traduction automatique

Ved anvendelse af en lipofil 1,1'-dioctadecy-3,3,3 ', 3'-tetramethylindocarbocyaninperchlorat (DiI) farvningsteknik kan Ambystoma-mexicanum gennemgå vaskulær perfusion for at muliggøre let visualisering af vaskulaturen.

Tags

Dil PerfusionVascular VisualizationAmbystoma MexicanumRegenerative MedicineAxolotlLimb RegenerationSedationThoracic CavityPericardiumAortaPeristaltic PumpPBSPerfusion

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