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Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
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1Max von Pettenkofer-Institut & Genzentrum, Virologie, Nationales Referenzzentrum für Retroviren, Medizinische Fakultät,LMU München, 2Institut für Laboratoriumsmedizin, klinische Chemie und Pathobiochemie,Charité – Universitätsmedizin Berlin, 3Institut für Physiologische Chemie,Martin-Luther-Universität Halle-Wittenberg
Chapitres
- 00:05Titre
- 01:02Synthesis of ManNProp and Related N-acyl-modefied Mannosamines
- 02:26Cell Culture and Cell Lysis for HPLC Analysis
- 04:28Separation of the Membrane Fraction and Acid Hydrolysis
- 05:27Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis
- 06:55Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells
- 09:00Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines
- 10:40Conclusion
Sialic acid is a typical monosaccharide-unit found in glycoconjugates. It is involved in a plethora of molecular and cellular interactions. Here we present a method to modify cell surface sialic acid expression using metabolic glycoengineering with N-acetylmannosamine derivatives.
